Concentration of Polar MFGM Lipids from Buttermilk by Microfiltration and Supercritical Fluid Extraction

Milk fat globule membrane isolated from buttermilk or whey cream and their lipid components inhibit infectivity of rotavirus in vitro.

Milk fat is encapsulated in a milk fat globule membrane (MFGM) that contains bioactive glycoproteins and glycolipids. The MFGM inhibits infectivity of rotavirus (RV), activity that has been attributed to its glycoprotein and carbohydrate components. However, previous studies of proteins and oligosaccharides in the MGFM have not accounted for all the bioactivity associated with the complete MFGM...

Strategies for supercritical fluid extraction of polar and ionic compounds

The methods used to facilitate the supercritical fluid extraction (SFE) of polar and ionic compounds are based on two general principles, either increasing the polarity of the supercritical fluid (SF) (usually CO,) used as extractant or reducing the polarity of the analytes to be leached from the solid matrices. Changes in the pressure and temperature of the SF, the use of SFs more polar than C...

Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry

A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAM...

Supercritical Fluid Extraction+supercritical Fluid Chromatography

high pressures also requires special equipment. In summary, both RESS crystallization and SAS crystallization appear to be promising methods for generating supersaturation and therefore represent alternatives to conventional crystallization. Such alternatives may prove attractive in applications such as polymer and pharmaceutical processing, or in particle design for drug delivery. It is possib...

Supercritical Fluid Extraction